Normal Lung Fibroblast Cell Line

The contributions from this cell line towards human virus vaccine production have been credited with avoiding disease in o.

Normal lung fibroblast cell line.

This is a normal diploid human cell line with 46 xy karyotype. It is an autocrine growth factor as well as a mitogen for astrocytes schwann cells and fibroblasts. Warm both the trypsin edta for primary cells atcc pcs 999 003 and the trypsin neutralizing solution atcc pcs 999 004 to room temperature prior to dissociation. For example 6 thio guanine 6 tg and 8 azaguanine 8 ag are cytotoxic to cell having functional hgprt.

Human fetal lung fibroblast cells mrc 5 line the mrc 5 cell line is commonly utilized in vaccine development as a transfection host in virology research and for in vitro cytotoxicity testing. Both x and y chromosomes were normal. The rate of polyploidy was 3 6. Jacobs the cell line was derived from the human lung tissue of a 14 week old male fetus aborted from a 27 year old woman.

Cell pellet in rnalater 1 000 000 cells. Chromosome frequency distribution 50 cells. The cell line isolated by leonard hayflick in the 1960s has been used extensively in scientific research with applications ranging from developing important theories in molecular biology and aging to the production of most human virus vaccines. Initiated in september 1966 by j.

The modal chromosome number was 46 occurring in 70 of cells. This hgprt gene encodes for the hgprt enzyme which is responsible for the conversion of the purines into nucleotides toxic purine analog such as nucleobase guanine. Cryopreserved clonetics tm normal human lung fibroblasts cells are guaranteed through 15 population doublings they are tested for negative von willebrand factor expression factor viii cytokeratins 18 and 19 and alpha smooth muscle actin all cells test negative for mycoplasma bacteria yeast and fungi. It is related to epidermal growth factor egf and transforming growth factor alpha tgf alpha.

Specially characterized normal human diploid fibroblast cultures this collection includes the imr 90 female and imr 91 male human fetal lung fibroblast lines which were developed expressly for the nia aging cell repository and are available at low intermediate and high population doublings. Selected product human pulmonary fibroblasts hpf. Some researcher use human normal lung fibroblasts hel 299 and mrc 5 cells while others use normal cell lines that don t belong to lung tissue. The chinese hamster lung fibroblast v 79 cell line is used to assess the mutagenic potential of chemical compounds inducing mutation at the heterozygous hgprt gene locus the gene that is present on the x chromosome.

Hiv 1 hepatitis b and hepatitis c are not detected for all donors and or cell lots. This protein interacts with the egf tgf alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. Cytogenetic information is based on initial seed stock at atcc.