One Step Rt Pcr Kit Thermo

Verso 1 step rt pcr kit supplied by thermo fisher used in various techniques.

One step rt pcr kit thermo.

99 100 based on 19 pubmed citations. Zero bias scores article reviews protocol conditions and more. Even after 4 hours at room temperature highly efficient and specific target amplification was achieved. This reduces the number of sample manipulations required and time to result but unlike two step strategies all of the cdna is used in the pcr so a cdna.

Agpath id one step rt pcr reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube minimizing sample handling errors and expediting set up time. Thermo fisher superscript iii platinum one step rt pcr chemistry the superscript iii one step rt pcr system with platinum taq dna polymerase is designed for the sensitive reproducible end point detection and analysis of rna molecules by rt pcr using this convenient one step formulation you can perform both cdna synthesis and pcr amplification in a single tube using gene specific primers and. Duplicate one step rt pcr reactions of 0 35 kb rna target from 1 and 10 pg of total hela rna were assembled using superscript iv one step rt pcr reagents and left at room temperature for up to 4 hours before cycling.

Thermo fisher agpath id one step rt pcr kit agpath id one step rt pcr reagents are designed for sensitive robust amplification of rna targets using a rapid single tube taqman real time reverse transcription pcr rt pcr strategy recommended for rna pathogen amplification consistent amplification of rna targets with high specificity and sensitivity optimized to work with your target.